کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7558753 1491379 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A universal homogeneous assay for high-throughput determination of binding kinetics
ترجمه فارسی عنوان
یک تست همگن جهانی برای تعیین توانایی کینتیک اتصال
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
There is an increasing demand for assay technologies that enable accurate, cost-effective, and high-throughput measurements of drug-target association and dissociation rates. Here we introduce a universal homogeneous kinetic probe competition assay (kPCA) that meets these requirements. The time-resolved fluorescence energy transfer (TR-FRET) procedure combines the versatility of radioligand binding assays with the advantages of homogeneous nonradioactive techniques while approaching the time resolution of surface plasmon resonance (SPR) and related biosensors. We show application of kPCA for three important target classes: enzymes, protein-protein interactions, and G protein-coupled receptors (GPCRs). This method is capable of supporting early stages of drug discovery with large amounts of kinetic information.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 468, 1 January 2015, Pages 42-49
نویسندگان
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