کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7558811 | 1491380 | 2014 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sensitive electrochemical measurement of hydroxyl radical generation induced by the xanthine-xanthine oxidase system
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A sensitive electrochemical measurement system for hydroxyl radical (OH) was developed using enzyme-catalyzed signal amplification. In the presence of 2,6-xylenol as a trapping agent, glucose as a substrate, and pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) as a catalyst, the amperometric signal of the trapping adduct 2,6-dimethylhydroquinone (DMHQ) produced by the hydroxylation of 2,6-xylenol was able to be amplified and detected sensitively. The limit of detection (signal/noise [S/N]Â =Â 3) for DMHQ was 1Â nM. There was no significant interference from urate and other oxidizable compounds in the reaction mixture at the applied potential of 0Â V versus Ag/AgCl. This method was employed to observe the OH generation induced by the xanthine-xanthine oxidase (XO) system. The reaction rates of the DMHQ production induced from the xanthine-XO system in the presence and absence of various Fe(III) complexes and proteins were compared. Those with a free coordination site on the Fe atom effectively enhanced the OH generation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 467, 15 December 2014, Pages 22-27
Journal: Analytical Biochemistry - Volume 467, 15 December 2014, Pages 22-27
نویسندگان
Hirosuke Tatsumi, Yui Tsuchiya, Koichi Sakamoto,