کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7559796 | 1491408 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of a high-throughput fluorescence polarization assay for the discovery of phosphopantetheinyl transferase inhibitors
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of a high-throughput fluorescence polarization assay for the discovery of phosphopantetheinyl transferase inhibitors Development of a high-throughput fluorescence polarization assay for the discovery of phosphopantetheinyl transferase inhibitors](/preview/png/7559796.png)
چکیده انگلیسی
An alarming number of clinically relevant bacterial pathogens are becoming resistant to many antibiotics, thereby fueling intense research into the discovery of novel therapeutic targets. Phosphopantetheinyl transferases (PPTases) represent a promising target for antibacterial development because these enzymes are crucial for the biosynthesis of a multitude of a pathogen's collection of essential metabolites and virulence factors biosynthesized via polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS) pathways. Here we describe the development of a fluorescence polarization (FP) assay that is amenable for high-throughput screening to identify PPTase inhibitors. The FP assay was validated against a panel of competitive ligands and displayed an excellent Zâ² score.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 403, Issues 1â2, August 2010, Pages 13-19
Journal: Analytical Biochemistry - Volume 403, Issues 1â2, August 2010, Pages 13-19
نویسندگان
Benjamin P. Duckworth, Courtney C. Aldrich,