Evaluating the use of HILIC in large-scale, multi dimensional proteomics: Horses for courses?

- HILIC provides a robust and reproducible off-line LC method for 2D-LC deep proteomics workflows.
- HILIC is part-orthogonal to hSAX fractionation.
- HILIC-based workflow identified ∼81,000 peptides from >9000 proteins with an average sequence coverage of ∼26%.
- HILIC is well suited for the study of hydrophilic PTMs.