Electron detachment/photodetachment dissociation of lasso peptides

- EDD and a-EPD reveal signatures of mechanically interlocked peptides.
- Lasso and non-lasso peptide topoisomers exhibit a different charge location.
- EDD and a-EPD of MccJ25 trigger mechanically interlocked two-peptide product ions.
- The two-peptide product ion [(c8)*(z2)] relies on an unknown stabilization mode.