کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7604344 1492240 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In-source decay characterization of isoaspartate and β-peptides
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
In-source decay characterization of isoaspartate and β-peptides
چکیده انگلیسی
Deamidation and the subsequent formation of isoaspartic acid (isoAsp) are common modifications of asparagine (Asn) residues in proteins. Differentiation of isoAsp and Asp residues is a challenging task owing to their similar chemical properties and identical molecular mass. Recent studies showed that they can be differentiated using electron capture dissociation (ECD) which generates diagnostic fragments c′ + 57 and z-57 specific to the isoAsp residue. However, the ECD approach is only applicable toward multiply-charged precursor ions and generally does not work for β-amino acids other than isoAsp. In this study, the potential of in-source decay (ISD) for characterization of isoAsp and other β-amino acids was explored. For isoAsp-containing peptides, ISD with a conventional hydrogen-donating matrix produced ECD-like c′ + 57 and z-57 diagnostic ions, even for singly charged precursor ions. For other β-amino acids, a hydrogen-accepting matrix was used to induce formation of site-specific a-14 ions from a synthetic β-analog of substance P. These results indicated that ISD can be broadly applied for β-peptide characterization.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Mass Spectrometry - Volume 390, 15 November 2015, Pages 101-109
نویسندگان
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