Dissociation of proton-bound complexes reveals geometry and arrangement of double bonds in unsaturated lipids

- cis- and trans-fatty acid stereoisomers can be difficult to distinguish by LC-MS.
- Proton-bound complexes of fatty acid methyl esters and iodoanilines generated.
- CID spectra of complexes are different for cis- and trans-fatty acids.
- Fragmentation involves carbene intermediates.