Automated detection and analysis of bimodal isotope peak distributions in H/D exchange mass spectrometry using HeXicon

▶ HeXicon was modified to specifically analyze bimodal isotope distributions in amide hydrogen exchange mass spectrometry datasets. ▶ In a dataset of a pulse-labeling HX-MS experiment the modified HeXicon was able to find all previously known peptides with bimodal isotope distribution. ▶ The modified HeXicon is able to identify previously unknown peptides with bimodal isotope distribution.