Cooperativity and allostery in cAMP-dependent activation of Protein Kinase A: Monitoring conformations of intermediates by amide hydrogen/deuterium exchange

▶ Amide H/D exchange to monitor intermediates in cAMP-dependent activation of PKA. ▶ cAMP binding to CNB-B partially disrupts R:C interface of PKA without dissociation. ▶ cAMP binding to CNB-B increases dynamics in interdomain helices. ▶ Basis for positive cooperativity in cAMP activation of PKA.