Extraction of chlorothalonil, dichlofluanid, DCOIT, and TCMTB from fish tissues employing the vortex assisted matrix solid-phase dispersion

This paper reports the development and optimization of a method based on vortex assisted matrix solid-phase extraction (VA-MSPD) and gas chromatography with electron capture detection for the evaluation of booster biocides in fish tissues. Some parameters that affect the method performance were evaluated and suitable recoveries were obtained with 0.2 g sample, 2.0 g reused end capped C18, 0.2 g sodium sulfate and 5 mL ethyl acetate (35 mmol L−1 acetic acid). After validation, the method was applied to both species Mugil liza and Cynoscion guatucupa and to different tissues of Micropogonias furnieri. Analytical recoveries ranged from 60 to 126% whereas relative standard deviation was below 18%. The limit of quantification value of chlorothalonil was 125 ng g−1 while the one of dichlofluanid, DCOIT and TCMTB was 625 ng g−1. The use of VA-MSPD for this proposal has shown to be a promising alternative since it is fast, simple and low cost. Besides, this is the first study that aims at extracting DCOIT and TCMTB from fish samples.