کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7640076 | 1494853 | 2018 | 26 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analyses of α-dicarbonyl compounds in food samples by HPLC using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine as a derivatizing reagent
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
α-Dicarbonyl compounds, such as 3-deoxyglucosone (3-DG), glyoxal (Gly), methylglyoxal (MGly), diacetyl (DA), and pentane-2,3-dione (PD) have been widely studied because they are the main precursors of advanced glycation end products. In this work, an analytical procedure was developed for the determination of these compounds by HPLC-UV using 4-(2,3-dimethyl-6-quinoxalinyl)-1,2-benzenediamine (DQB) as a novel derivatizing reagent. After derivatization at pHâ¯9.0 and ambient temperature for 30â¯min, these five compounds were analyzed by HPLC. The results showed that the linear correlation coefficients (R2) of the method for the five analytes are between 0.994 and 0.999 in broad concentration ranges, the limits of detection are 0.050â¯Î¼M (3-DG), 0.050â¯Î¼M (Gly), 0.050â¯Î¼M (MGly), 0.020â¯Î¼M (DA), and 0.025â¯Î¼M (PD), respectively. The method was further evaluated in the analyses of many kinds of food samples employing the standard addition method with the RSDs ranging from 0.43 to 4.07% and the recoveries from 85% to 103%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 141, September 2018, Pages 64-70
Journal: Microchemical Journal - Volume 141, September 2018, Pages 64-70
نویسندگان
Xin-Jie Wang, Feng Gao, Lu-Chao Li, Xian Hui, Heng Li, Wen-Yun Gao,