کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
7641308 1494864 2017 29 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a quantitative assay for the determination of cinacalcet and its main metabolites in human plasma using RP-HPLC method
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development and validation of a quantitative assay for the determination of cinacalcet and its main metabolites in human plasma using RP-HPLC method
چکیده انگلیسی
A novel and sensitive methodology for the quantitative determination of cinacalcet (CIN), and its main metabolites (M2a-Glu, M2b-Glu, M5, and M6) in plasma sample is suggested. The analytical assay is based on reversed-phase high-performance liquid chromatography (RP-HPLC) coupled with a fluorescence detector. The method has been fully validated at range covering the plasma concentrations in patients receiving therapeutic dosages of CIN. In this investigation, 2-(2,3-naphthalimino)ethyl trifluoromethanesulfonate (NI-ETFMS) was used as a pre-column derivation agent according to interaction of amine and carboxylate groups of CIN and its metabolites with NI-ETFMS. The sample pretreatment consists of protein precipitation with acetonitrile using only 100 μL of plasma, and chromatographic separation was achieved in isocratic mode on Kinetex C18 100 Å column with mobile phase of acetonitrile, methanol, and 50 mM phosphate buffer (40:20:40 v/v). The analytical method was fully validated in terms of selectivity, linear range (0.2 to 5.5 ng mL− 1), linearity (r2 > 0.997), sensitivity (LOD = 0.05 to 0.75 ng mL− 1 and LOQ = 0.36 to 2.52 ng mL− 1), intra and inter-days accuracy (− 4.6 to 8.1%), precision (< 7.4%), and robustness (< 3.5%). As results, the proposed method can be useful in the routine analysis for the determination of CIN and its main metabolites in human plasma samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 130, January 2017, Pages 377-383
نویسندگان
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