کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7642852 | 1494877 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expanding the separation capability of sequential injection chromatography: Determination of melamine in milk exploiting micellar medium and on-line sample preparation
ترجمه فارسی عنوان
گسترش قابلیت جداسازی کروماتوگرافی تزریق ترتیبی: تعیین ملامین در بهره برداری از شیر میسل و آماده سازی نمونه های آنلاین
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کلمات کلیدی
کروماتوگرافی تزریقی متوالی، کروماتوگرافی میکسل، ستون های یکپارچه، ملایم، شیر،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Silica, C8 and C18 monolithic column were the only option for separation on a sequential injection chromatography (SIC) system due its low-pressure operation. Therewith, the separation of molecules poorly retained by this stationary phase was a difficult task. This work presents the first use of micellar chromatography on a SIC system, with the applicability demonstrated by the determination of melamine in milk. The mobile phase was composed by aqueous sodium dodecyl sulfate (SDS) solution and propanol (92.5:7.5). The adsorption of SDS monomers on the C18 stationary phase modified its chromatographic properties, enabling satisfactory melamine separation from the milk components. The sample pretreatment procedure was on-line implemented by dilution of the sample with SDS using a multiposition valve. Calibration equations obtained from melamine solutions prepared in different types of milk and water presented similar sensitivity, indicating absence of matrix effects. A linear response was observed within 2.0 and 6.0 mg Lâ 1 of melamine with a detection limit estimated at 0.6 mg Lâ 1 and coefficients of variation at 2.9% (n = 6). The procedure was suitable for fast determination of melamine below the limits established by FDA and WHO (2.5 mg Lâ 1), with minimized reagent consumption. Results for different milk samples agreed with those obtained by high performance liquid chromatography at the 95% confidence level.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 117, November 2014, Pages 106-110
Journal: Microchemical Journal - Volume 117, November 2014, Pages 106-110
نویسندگان
Alex D. Batista, Carina F. Nascimento, Wanessa R. Melchert, Fábio R.P. Rocha,