A two-component post-column derivatisation method utilising reaction flow chromatography

“Reaction flow (RF) chromatography” has recently emerged from exploiting a new column “Parallel Segmented Flow” (PSF) to post-column derivatisation (PCD) methods [1]. The main advantage RF has over conventional PCD methods is the elimination of large volume reaction coils, as mixing occurs in the PSF column outlet fitting design, therefore minimising peak broadening. In this investigation we take an existing PCD method for the detection of phenols and apply it to RF. The detection sensitivity of the RF method is discussed, with the results demonstrating that through careful control of the mobile phase and derivatisation reagent conditions the selectivity of the PCD reaction may target compounds other than phenols.