کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7643030 | 1494879 | 2014 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC-MS/MS
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC-MS/MS Development and validation of a multi-residue method for determination of 18 β-agonists in bovine urine by UPLC-MS/MS](/preview/png/7643030.png)
چکیده انگلیسی
Ultra performance liquid chromatography (UPLC) hyphenated to tandem mass spectrometry (MS/MS) was used for the development of an analytical method capable of simultaneous identification and quantification of 18 β-agonist compounds namely, brombuterol, chlorbrombuterol, cimaterol, cimbuterol, clenbuterol, clencyclohexerol, clenisopenterol, clenpenterol, clenproperol, hydroxymetylclenbuterol, isoxsuprine, mabuterol, mapenterol (clenbuterol-like compounds), ractopamine, ritodrine, salbutamol, salmeterol (salbutamol-like compounds) and zilpaterol in bovine urine. In compliance with the Commission Decision 2002/657/EC (CD 2002/657/EC), the method was validated applying a matrix-comprehensive in-house validation approach based on a fractional factorial design. Six experimental factors varied on two levels were selected for this purpose. The relevant validation parameters such as decision limit CCα (range, 0.24-0.51 μg Lâ 1) and detection capability CCβ (range, 0.27-0.71 μg Lâ 1), within-laboratory reproducibility (< 20%) as well as recovery (range, 92-109%) were in agreement with the performance criteria set in the CD 2002/657/EC. Overall, the proposed method enabled both screening and confirmatory detection of the β-agonist compounds in the framework of official monitoring plans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Microchemical Journal - Volume 115, July 2014, Pages 70-77
Journal: Microchemical Journal - Volume 115, July 2014, Pages 70-77
نویسندگان
D. Mauro, S. Ciardullo, C. Civitareale, M. Fiori, A.A. Pastorelli, P. Stacchini, G. Palleschi,