Transport of selenium species in Caco-2 cells: Analytical approach employing the Ussing chamber technique and HPLC-ICP-MS

► A simple and fast HPLC-ICP-DRC-MS method was developed for analysis of Se species. ► Se species were added to the Caco-2 cell model either simultaneously or separately. ► Selenite transport across Caco-2 cell monolayers is especially slow. ► Presence of other Se species influences the transport of selenate, SeMet, and MeSEC.