Prediction of mold contamination from microbial volatile organic compound profiles using solid phase microextraction and gas chromatography/mass spectrometry

► A new sampling and analytical methodology based on an integrated chemical and microbiological approach was developed to assess the fungal load of a contaminated area or building. ► A microbial VOC profile indicative of the degree of contamination of an indoor environment by fungal molds can measured using SPME and GC/MS. ► GC profiles of microbial VOCs sampled by SPME can be correlated to Anderson impactor data using discriminant analysis.