On-line chiral analysis of benzylmercapturic acid and phenylmercapturic acid in human urine using UPLC-QToF mass spectrometry with the kinetic method

► A novel on-line chiral analysis of biomarkers in flow system by using UPLC-QToF-MS system. ► Rapid quantitative chiral analysis of PMA and BMA with a modified kinetic method. ► Determine the enantiomeric composition in urine samples. ► Showed a good agreement between actual and the measurement value.