کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7699027 | 1496640 | 2017 | 27 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of picomolar dissolved free amino acids along a South Atlantic transect using reversed-phase high-performance liquid chromatography
ترجمه فارسی عنوان
تعیین اسیدهای آمینه رایگان حل شده پیکومولار در امتداد قطب جنوب اقیانوس اطلس با استفاده از کروماتوگرافی مایع با عملکرد بالا
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
چکیده انگلیسی
Dissolved free amino acids (DFAA) in seawater are a form of nitrogen (N) available for marine microbes. In oligotrophic environments where N-containing nutrients are the limiting factor for microbial growth, N nutrition from DFAA could be crucial, but as yet it is poorly resolved. Measurements of individual DFAA are challenging as concentrations are typically in the low nmol Lâ 1 range. Here we report modifications to methodology using o-phthaldialdehyde (OPA) derivatization and reversed phase high performance liquid chromatography (HPLC) that provide a 30-fold improvement in sensitivity enabling the detection of 15 amino acids in seawater with a limit of detection as low as 10 pmol Lâ 1 with accuracy and precision of better than 10%. This analytical methodology is now suitable for the challenging quantitation of DFAA in oligotrophic seawaters. The method was successfully applied to a suite of seawater samples collected on a cruise crossing the South Atlantic Ocean, where concentrations of DFAAs were generally low (sub nmol Lâ 1), revealing basin-scale features in the oceanographic distributions of DFAA. This unique dataset implies that DFAAs are an important component of the N cycle in both near-coastal and open oceans. Further calculations suggest that the proportions of organic N originating from DFAA sources were significant, contributing between 0.2 and 200% that of NH4+ and up to 77% that of total inorganic nitrogen in the upper 400 m in some regions of the transect.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Marine Chemistry - Volume 196, 20 November 2017, Pages 173-180
Journal: Marine Chemistry - Volume 196, 20 November 2017, Pages 173-180
نویسندگان
A.J.M. Sabadel, T.J. Browning, D. Kruimer, R.L. Airs, E.M.S. Woodward, R. Van Hale, R.D. Frew,