Study on regeneration effect and mechanism of high-frequency ultrasound on biological activated carbon

High frequency ultrasonic radiation technology was developed as a novel and efficient means of regenerating spent biological activated carbon (BAC) used in drinking water treatment plants (DWTPs). The results of this study indicated that high frequency ultrasonic treatment could recover the spent BAC, to some extent, with the following optimal conditions: a frequency of 400 kHz, sonication power of 60 W, water temperature of 30 °C, and sonication time of 6 min. Under the above conditions, the iodine value increased from 300 mg/g to 409 mg/g, the volume of total pores and micropores increased from 0.2600 cm3/g and 0.1779 cm3/g to 0.3560 cm3/g and 0.2662 cm3/g, respectively; the specific surface area of micropores and the mean pore diameter expanded from 361.15 m2/g and 2.0975 nm to 449.92 m2/g and 2.1268 nm, respectively. The biological activity increased from 0.0297 mgO2/gC·h to 0.0521 mgO2/gC·h, while the biomass decreased from 203 nmolP/gC to 180 nmolP/gC. The results of high throughput 16S rRNA gene amplicon sequencing showed that microorganisms such as Clostridia and Nitrospira were markedly decreased due to high frequency ultrasound. The method used in this study caused the inhibition of certain carbon-attached microbials resulting in a negative effect on the removal rate of ammonia-N during the initial stage of the long-term reuse operation. The removal of UV254 and atrazine were restored from 8.1% and 55% to 21% and 76%, respectively.