کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7702964 | 1496862 | 2018 | 35 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ultrasound-assisted d-tartaric acid whole-cell bioconversion by recombinant Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
d-Tartaric acid has wide range of application in the pharmaceutical industry and scarcely exists in nature. In this study, cis-epoxysuccinate hydrolase (CESH)-containing Escherichia coli was used to perform whole-cell bioconversion of cis-epoxysuccinate (CES) to D-tartaric acid and the catalytic efficiency was investigated by ultrasound treatment. The bioconversion rate of CES sodium reached 70.36% after 60â¯min treated after ultrasound, which is 3-fold higher than that in the control. The specific rate could be further improved by 2-fold after 5 repeated batches compared with the first one, however, the specific rate gradually decreased with the increase of repeat batches (>5 batches). The CESH from Bordetella sp. BK-52 was a typical Michaelis-Menten enzyme with Vmax and Km values of 28.17â¯mM/h/g WCW (wet of cell weight) and 30.18â¯mM, respectively. The process for the d-tartaric acid bioconversion, which consisted of 102.31â¯g/L CES sodium, 8.78â¯mg/mL whole cell and ultrasound power of 79.36â¯W, is further optimized using response surface methodology. The specific rate finally reached 194.79â¯Â±â¯1.78â¯mM/h/g WCW under the optimal conditions. Furthermore, the permeability of inner and outer membrane was improved approximately 1.6 and 1.4-fold after ultrasound treatment, respectively, which may be a crucial factor for improvement of the bioconversion efficiency.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics Sonochemistry - Volume 42, April 2018, Pages 11-17
Journal: Ultrasonics Sonochemistry - Volume 42, April 2018, Pages 11-17
نویسندگان
Weiliang Dong, Fenglian Zhao, Fengxue Xin, Aiyong He, Yue Zhang, Hao Wu, Yan Fang, Wenming Zhang, Jiangfeng Ma, Min Jiang,