کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7704221 | 1496877 | 2015 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Use of Adaptive Focused Acoustics⢠ultrasound in controlling liposome formation
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کلمات کلیدی
PBF1,2-distearoyl-sn-glycero-3-phosphoethanolamine-n-[methoxy(polyethylene glycol)-2000]MLVsLUVsHSPCPDIRCRCPBCHOLPBSmultilamellar vesicles - vesicles multilamellarlarge unilamellar vesicles - بزرگ کیسه های بدون انعطاف پذیرpolydispersity index - شاخص پلییدریزاییDuty factor - فاکتور وظیفهphosphate buffer saline - فسفات بافر شورAfa - پدر بزرگPiP - پیپcholesterol - کلسترول
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Many techniques for producing large unilamellar vesicles (LUVs) or small unilamellar vesicles (SUVs) have drawbacks, including exposure of sensitive biological materials to harsh organic solvents or high temperatures. Here we describe the use of controlled focused ultrasound, Adaptive Focused Acoustics⢠(AFA), to make LUV or SUV at low temperature without organic solvents and at a consistent, chosen size. We studied the effects of peak incident power (PIP), cycles per burst (CPB), duty factor (DF), temperature, and lipid composition (natural or synthetic), on liposome size distribution. We found that an increase in PIP, DF, CPB, or temperature decreased liposome size. When processed under the same conditions as the natural lipid composition [Phospholipon 90 G], the synthetic lipid composition [HSPC, DSPE-PEG-2000, Chol] generally produced larger liposomes, although extending processing time reduced liposomes to similar size. In combination with AFA, these trends can help pinpoint parameter values that achieve a desired liposome size distribution.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Ultrasonics Sonochemistry - Volume 27, November 2015, Pages 638-645
Journal: Ultrasonics Sonochemistry - Volume 27, November 2015, Pages 638-645
نویسندگان
Katherine C. Shen, Srikanth Kakumanu, Carl D. Beckett, James A. Jr.,