Nucleotide divergence and genetic relationships of Pseudoroegneria species

► We characterized nucleotide variation in 8 diploid Pseudoroegneria species. ► The highest nucleotide diversity of the RPB2 was found for Pseudoroegneria spicata among the taxa analyzed. ► A close relationship among P. spicata, Pseudoroegneria gracillima and Pseudoroegneria stipifolia was revealed. ► Pseudoroegneria libanotica and Pseudoroegneria tauri were also found to exhibit a high level of sequence homology.