The synthesis, antiviral, cytostatic and cytotoxic evaluation of a new series of acyclonucleotide analogues with a 1,2,3-triazole linker

The 1,2,3-triazoloacyclonucleotides were evaluated in vitro for activity against a broad variety of DNA and RNA viruses and cytostatic activity against murine leukaemia L1210, human T-lymphocyte CEM and human cervix carcinoma HeLa cells. Diethyl 3-{4-[(3-benzoyl-2,4-dioxoquinazolin-1-yl)methyl]-1H-1,2,3-triazol-1-yl}propylphosphonate exhibited activity against both herpes simplex viruses (HSV-1, HSV-2) in HEL cell cultures (EC50 = 17 μM) and feline herpes virus (EC50 = 24 μM) in CRFK cell cultures. Several compounds preferentially inhibited proliferation of human T-lymphocyte CEM cells at IC50 in the 2.8-12 μM range.