Mannanase Enzyme from Bacillus subtilis P2-5 with Waste Management

Nowadays, waste disposal has become a major of environment problem in Thailand because the volume of waste increase due to population growth and social behavior. It required optimal solutions in waste management. People should realize fundamental of 3R including reduce recycle and reuse in order to improve environment condition. This research interested in coffee grounds and tea waste because they were generated from household and industrial thus require to disposal or added value. The objective of this research was recycle coffee grounds and tea waste by utilize as a raw material for mannanase enzyme production. Mannanase enzyme can hydrolyze mannan structure, containing in plant such as konjac, ivory nut, locust bean gum, guar gum, palm kernel, coffee bean and copra meal. The methodology was a randomized complete block design to studied mannanase enzyme activity in different condition. There were two factors consist of inoculum percentage; Bacillus subtilis P2-5 had three levels (1, 3 and 5% (v/v) and three sources (locust bean gum, coffee grounds and tea waste) in producing medium (PM) for mannanase production. An analysis of variances (ANOVA) method was applied to determine the effect of inoculum percentage and regression analysis to predict mannanase enzyme activity. The test result found that inoculum percentage and three sources significantly affecting the mannanase enzyme activity. The prediction model had been formulated and prioritize source which produce the high mannanase enzyme activity as following tea waste, coffee grounds and locust bean gum, respectively. This research would be guidelines for waste management strategy to achieve sustainability approach.