کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
827320 907994 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simultaneous HPLC quantitative analysis of mangostin derivatives in Tetragonula pagdeni propolis extracts
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Simultaneous HPLC quantitative analysis of mangostin derivatives in Tetragonula pagdeni propolis extracts
چکیده انگلیسی

Propolis has been used as indigenous medicine for curing numerous maladies. The one that is of ethnopharmacological use is stingless bee propolis from Tetragonula pagdeni. A simultaneous high-performance liquid chromatography (HPLC) investigation was developed and validated to determine the contents of bioactive compounds: 3-isomangostin, gamma-mangostin, beta-mangostin, and alpha-mangostin. HPLC analysis was effectively performed using a Hypersil BDS C18 column, with the gradient elution of methanol–0.2% formic acid and a flow rate of 1 ml/min, at 25 °C and detected at 245 nm. Parameters for the validation included accuracy, precision, linearity, and limits of quantitation and detection. The developed HPLC technique was precise, with lower than 2% relative standard deviation. The recovery values of 3-isomangostin, gamma-mangostin, beta-mangostin, and alpha-mangostin in the extracts were 99.98%, 99.97%, 98.98% and 99.19%, respectively. The average contents of these mixtures in the propolis extracts collected from different seasons were 0.127%, 1.008%, 0.323% and 2.703% (w/w), respectively. The developed HPLC technique was suitable and practical for the simultaneous analysis of these mangostin derivatives in T. pagdeni propolis and would be a valuable guidance for the standardization of its pharmaceutical products.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of King Saud University - Science - Volume 28, Issue 2, April 2016, Pages 131–135
نویسندگان
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