کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8274230 | 1535097 | 2016 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neurotransmitter and their metabolite concentrations in different areas of the HPRT knockout mouse brain
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کلمات کلیدی
DHPG5-HIAAHprt5-Hydroxyindoleacetic acid - 5-هیدروکسی سدیم اسیدHILIC - هیلیکhypoxanthine-guanine phosphoribosyltransferase - هیپوکسانتین-گوانین فسفریبوسیل ترانسفرازhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاhydrophilic interaction liquid chromatography - کروماتوگرافی مایع متقاطع هیدروفیلیHPLC - کروماتوگرافی مایعی کاراGABA - گابا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Neurotransmitter and their metabolite concentrations in different areas of the HPRT knockout mouse brain Neurotransmitter and their metabolite concentrations in different areas of the HPRT knockout mouse brain](/preview/png/8274230.png)
چکیده انگلیسی
Lesch-Nyhan syndrome (LNS) is characterized by uric acid overproduction and severe neurobehavioral symptoms, such as recurrent self-mutilative behavior. To learn more about the pathophysiology of the disease, we quantified neurotransmitters and their metabolites in the cerebral hemisphere, cerebellum and the medulla oblongata of HPRT knockout mice, an animal model for LNS, in comparison to the corresponding wild-type. Our analyses included l-glutamate, 4-aminobutanoic acid (GABA), acetylcholine, serotonin, 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine, l-normetanephrine, epinephrine and l-metanephrine and were conducted via high performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS/MS). Among these neurotransmitter systems, we did not find any abnormalities in the HPRT knockout mouse brains. On one side, this might indicate that HPRT deficiency most severely affects dopamine signaling, while brain functioning based on other neurotransmitters is more or less spared. On the other hand, our findings may reflect a compensating mechanism for impaired purine salvage that protects the brain in HPRT-deficient mice but not in LNS patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Neurological Sciences - Volume 365, 15 June 2016, Pages 169-174
Journal: Journal of the Neurological Sciences - Volume 365, 15 June 2016, Pages 169-174
نویسندگان
Sarah K. Tschirner, Frank Gutzki, Erich H. Schneider, Roland Seifert, Volkhard Kaever,