کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8274990 1535101 2016 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Initial research on the relationship between let-7 family members in the serum and massive cerebral infarction
ترجمه فارسی عنوان
تحقیق اولیه در مورد ارتباط بین اعضای خانواده اجازه 7 در سرم و انفارکتوس بزرگ مغزی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
چکیده انگلیسی
Eighty-eight ischemic stroke patients with massive cerebral infarction (MCI) who met our selection criteria were included in this study. MCI was assessed using the Glasgow Coma Scale (GCS) at hospital admission and at 2 weeks. The sera of all patients and controls were sampled at 48 h after the patients' attacks, and the sera of patients with MCI who had no severe cardiopulmonary complications, including those with hemorrhagic transformation (HT), were sampled again at 2 weeks. The relative expression of let-7 miRNA in the serum was determined by real-time qRT-PCR, and the blood levels of lipids, glucose, high-sensitivity C-reactive protein (hs-CRP), homocysteine and blood pressure were measured at admission. Interleukin-6 (IL-6) levels were detected by ELISA, and a luciferase assay was performed to confirm that IL-6 was a gene target of let-7. The relative expression of let-7f was significantly down-regulated in MCI without HT patients compared with controls (P < 0.001), and it was positively correlated with GCS (P < 0.01) and negatively correlated with hs-CRP (P < 0.01). The relative expression of let-7f was significantly up-regulated in MCI patients with HT (P < 0.01). IL-6 is a direct target gene for let-7f, and IL-6 expression was increased in MCI without HT patients compared to controls (P < 0.01). The expression of let-7f in serum is associated with MCI without HT, which specifically inhibits IL-6. This suggests that let-7f may control inflammation in patients with MCI without HT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of the Neurological Sciences - Volume 361, 15 February 2016, Pages 150-157
نویسندگان
, , , , , , , , , , ,