کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8287553 | 1535857 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Diabetes mellitus- induction: Effect of different streptozotocin doses on male reproductive parameters
ترجمه فارسی عنوان
دیابت نوع دوم: اثر دوزهای استرپتوزوتوسین مختلف بر پارامترهای تولید مثل مردان
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
Adult male Wistar rats received either a single intraperitoneal injection of STZ (30â¯mg/kg or 60â¯mg/kg) or saline (0.9%, Control). Diabetes was confirmed after 72â¯h if plasma glucose levels were â¥14â¯mmol/L. Body weight, glucose level, fluid and food intake were measured weekly. Animals were sacrificed after 8 weeks of treatment by an overdose of sodium pentobarbital (160â¯mg/kg body weight). The testis and epididymis were harvested and weighed prior to preparation for histological evaluation. Epididymal sperm morphology was analysed using computer aided sperm analysis (CASA). STZ60 animals presented with significantly lower body weights compared to both control and STZ30 groups. Animals in both STZ30 and STZ60 groups showed decreased normal sperm morphology compared to control. Histological evaluation of the testes showed a decrease in the number of spermatozoa in the seminiferous tubules of animals in the STZ30 and STZ60 groups compared to control. A complete absence of spermiogenesis was observed in the seminiferous tubules of STZ60 animals. These findings prove that an STZ concentration of 30â¯mg/kg, which is much lower than the reported 40â¯mg/kg, has adverse effects on the male reproductive system via its diabetogenic effect and can therefore be used to study the impact of DM on male fertility.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 120, Issue 2, February 2018, Pages 103-109
Journal: Acta Histochemica - Volume 120, Issue 2, February 2018, Pages 103-109
نویسندگان
Temidayo S. Omolaoye, Bongekile T. Skosana, Stefan S. du Plessis,