کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8318261 | 1538969 | 2018 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Activation of free sperm and dissociation of sperm bundles (spermatozeugmata) of an endangered viviparous fish, Xenotoca eiseni
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Knowledge of sperm motility activation for viviparous fishes has been limited to study of several species in Poeciliidae, and the dissociation of sperm bundles is even less understood. The goal of this study was to use the endangered Redtail Splitfin (Xenotoca eiseni) as a model to investigate the activation of sperm from viviparous fishes by study of free sperm and spermatozeugmata (unencapsulated sperm bundles). The specific objectives were to evaluate the effects of: (1) osmotic pressure and refrigerated storage (4â¯Â°C) on activation of free sperm, (2) osmotic pressure, ions, and pH on dissociation of spermatozeugmata, and (3) CaCl2 concentration and pH on sperm membrane integrity. Free sperm were activated in Ca2+-free Hanks' balanced salt solution at 81-516â¯mOsmol/kg. The highest motility (19â¯Â±â¯6%) was at 305â¯mOsmol/kg and swim remained for 84â¯h. Glucose (300-700â¯mOsmol/kg), NaCl (50-600â¯mOsmol/kg), and KCl, MgCl2, and MnCl2 at 5-160â¯mM activated sperm within spermatozeugmata, but did not dissociate spermatozeugmata. CaCl2 at 5-160â¯mM dissociated spermatozeugmata within 10â¯min. Solutions of NaCl-NaOH at pHâ¯11.6 to 12.4 dissociated spermatozeugmata within 1â¯min. The percentage of viable cells had no significant differences (Pâ¯=â¯0.2033) among different concentrations of CaCl2, but it was lower (Pâ¯<â¯0.0001) at pHâ¯12.5 than at pH between 7.0 and 12.0. Overall, this study provided a foundation for quality evaluation of sperm and spermatozeugmata from livebearing fishes, and for development of germplasm repositories for imperiled goodeids.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 218, April 2018, Pages 35-45
Journal: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology - Volume 218, April 2018, Pages 35-45
نویسندگان
Yue Liu, Huiping Yang, Leticia Torres, Terrence R. Tiersch,