Development of a CCK1R-membrane nanoparticle as a fish-out tool for bioactive peptides

- Cholecystokinin receptor 1 (CCK1R) was functionally expressed in HEK-293T cells.
- Heterogeneously expressed CCK1R and zebrafish apo-lipoprotein were purified.
- Formation of the CCKR1-NABB (nanoscale apo-lipoprotein bound bilayer) particles.
- CCK-8 facilitates the binding of fluorescently labeled CCK to CCK1R-NABBs.