کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8362983 1542566 2018 12 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phosphate levels influence the utilisation of rice rhizodeposition carbon and the phosphate-solubilising microbial community in a paddy soil
ترجمه فارسی عنوان
سطوح فسفات بر استفاده از کربن ریززدایی برنج و جامعه میکروبی حلالیت فسفات در خاک بارشی تاثیر می گذارد
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش خاک شناسی
چکیده انگلیسی
To fulfil the demand of a growing population for rice food, a better understanding of soil biological systems is urgently required to improve the efficient use of chemical fertilisers, especially phosphate (P)-based fertilisers. Microorganisms play an important role in the decomposition of organic compounds and solubilisation of inorganic P. Presently, whether P availability affects the incorporation of rice root-derived carbon and the activity of P-solubilising bacteria remains uncertain. Herein, stable isotope probing of phospholipid fatty acids and DNA and high-throughput sequencing methods were used to investigate active P-solubilising bacteria and the microbial community in a paddy soil treated at 0 (controls), 50, and 200 mg P2O5 kg−1. Compared with controls (23.4-62.2%), the δ13C values of 16:1ω7c, 18:1ω7c, and 18:1ω9c (Gram-negative bacteria) increased to 69.2-167.4% with low P input, but decreased to 6.6-58.6% under high P input. Similarly, higher 13C enrichment (1.04-1.47 ng g−1 dry weight soil) was observed in phospholipid fatty acids (16:1ω7c, 18:1ω7c and 18:1ω9c) with low P input. High-throughput sequencing indicated that low rather than high P input promoted the growth of P-solubilising Bacillus, Alicyclobacillus, and Clostridium. The relative abundance of these organisms increased significantly in light DNA fractions following both 12C and 13C treatments, suggesting they seldom use rice root-derived carbon as carbon sources. By contrast, P-solubilising Rhizobiales, Rhodospirillales, and Myxococcales preferred root-derived carbon under P input conditions. Community and phylogenetic analysis of genes related to P solubilisation (alkaline phosphatase phoD and pyrroloquinoline quinone pqqC) indicated a dominant role for Rhizobiales and Actinomycetales in paddy soil P solubilisation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Soil Biology and Biochemistry - Volume 118, March 2018, Pages 103-114
نویسندگان
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