Soil microbial biomass and its activity estimated by kinetic respiration analysis - Statistical guidelines

► Guide kinetic respiration analysis for estimating microbial biomass and activity. ► Suggest graphical depictive rule to constrain the exponential growth phase. ► Evaluate objective statistical criteria to constrain the exponential growth phase. ► Maximum weighted r2 does best if nutrient amendment has been complete. ► Discuss uncertainty, bias, and spacing of respiration measurements.