کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8369108 | 1543032 | 2018 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distinct effect of fetal bovine serum versus follicular fluid on multipotentiality of human granulosa cells in in vitro condition
ترجمه فارسی عنوان
اثر متمایز سرم جنین گاوی در برابر مایع فولیکولی بر چندعاملیت سلول های گرانولوزا انسان در شرایط آزمایشگاهی
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کلمات کلیدی
سلول های گرانولوزا انسانی، مایع فولیکولار، سرم جنین گاو، چند منظوره،
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
This study aimed to develop an appropriate medium for preservation of multipotentiality in human granulosa cells. To compare the possible effect of different media supplemented with follicular fluid or fetal bovine serum, granulosa cells were cultured in vitro over a period of 14 days. Stemness feature and any alteration in the cell phenotype were monitored using colony count assay and flow cytometry analysis by monitoring the expression of Oct3/4 and GATA-4 factors. Transcript expression level of Sox-2, Klf-4, and Nanog were investigated using quantitative real-time PCR analysis. Cells were cultured in the medium supplement with follicular fluid showed normal cell morphology and epithelial-like appearance, however, cells treated with fetal bovine serum, exhibited the clonogenic potential of granulosa cells which was increased after exposure to follicular fluid after 14 days (pâ¯<â¯0.05). Flow cytometry analysis revealed a significant reduction in the protein level of GATA-4 in cells cultured in presence of follicular fluid compared with cells received fetal bovine serum (pâ¯<â¯0.001). Quantitative real-time PCR analysis disclosed reduction of Sox-2, Klf-4 and Nanog levels in cells exposed to fetal bovine serum. Our experiment showed the exposure of human granulosa cells to follicular fluid efficiently preserves the stemness characteristics of the cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 52, March 2018, Pages 44-48
Journal: Biologicals - Volume 52, March 2018, Pages 44-48
نویسندگان
Soudabe Yousefi, Jafar Soleimanirad, Kobra Hamdi, Laya Farzadi, Aalie Ghasemzadeh, Masoumeh Kazemi, Mahdi Mahdipour, Reza Rahbarghazi, Mohammad Nouri,