کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8369176 1543034 2017 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding and neutralizing anti-cytomegalovirus activities in immune globulin products
ترجمه فارسی عنوان
فعالیت های ضد سیتومگالوویروس مرتبط و غیرفعال شده در محصولات ایمنی گلوبولین
کلمات کلیدی
سیتومگالوویروس، گلوبولین ایمنی، آزمایش آنزیم ایمونوسیورسانس، خنثی سازی ویروس،
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
Congenital infection as well as infection of immunocompromised individuals by cytomegalovirus (CMV) can be associated with significant morbidity, mortality, and long-term adverse health outcomes. Assessment of anti-viral activity using appropriate assays is essential for ensuring safe and efficacious use of therapeutic CMV immune globulin (IG) products. In this study, we used commercial ELISA kits to compare anti-CMV antibody binding activity and avidity for lots of CMV-specific and normal IG products available in the US market. Additionally, neutralizing activity of IG products was measured against CMV strains (AD169wt131 or TB40E-GFP) in MRC-5 human fibroblasts and ARPE-19 human epithelial cells. Our data revealed that, regardless of the method, anti-CMV activity was higher in CMV IG lots we tested compared with normal IG lots; CMV binding activity was at least 4-fold higher, and neutralizing activity at least 2- and 3-fold higher for epithelial and fibroblast cells, respectively, in CMV IG lots compared with normal IG lots. Furthermore, anti-CMV activity values from all three methods (ELISA, neutralization in MRC-5 cells, and neutralization in ARPE-19 cells) were highly correlated, whereas avidity, although higher in CMV IG lots, did not correlate well with either binding or neutralizing activities.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 50, November 2017, Pages 35-41
نویسندگان
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