کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8369196 1543034 2017 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of marker gene expression as a potential predictive marker of leukopenic toxicity for inactivated influenza vaccines
ترجمه فارسی عنوان
ارزیابی بیان ژن مارکر به عنوان نشانگر پیش بینی کننده بالقوه سمیت لکوپنیک برای واکسن های غیرفعال شده آنفلوانزا
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
The leukopenic toxicity test (LTT) is used to evaluate the safety and lot-to-lot consistency of influenza hemagglutinin split vaccine (HAv) and is included in the Japanese Minimum Requirements for Biological Products. LTT assesses the reduced leukocyte levels in murine peripheral blood after HAv administration. However, they require large numbers of animals, and therefore it would be beneficial to develop a more accurate and sensitive alternative method. In this study, we selected biomarkers of leukocyte reduction from 18 previously identified marker genes that were associated with an abnormal toxicity test (ATT). Among these 18 genes, the expressions of 15 marker genes were strongly associated with leukocyte reduction levels. A stepwise single addition multiple regression analysis was used to further extract the genes responsible for leukocyte reduction, with significant (p < 0.25) regression coefficients. The expression of 7 genes significantly predicted the leukocyte reduction. The prediction accuracy of this approach was approximately >90% (mean) for the direct measurement of leukocyte numbers. These results indicate that the expression of these 18 previously identified genes can provide information for both ATT and LTT.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 50, November 2017, Pages 100-108
نویسندگان
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