کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8384872 | 1543664 | 2018 | 21 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Subdivision of MRSA CC398 isolates using MALDI-TOF MS
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Subdivision of MRSA CC398 isolates using MALDI-TOF MS Subdivision of MRSA CC398 isolates using MALDI-TOF MS](/preview/png/8384872.png)
چکیده انگلیسی
Outbreak investigations demand a fast and discriminative typing method. MALDI-TOF MS has been shown to be a rapid, easy and inexpensive method of subtyping MRSA.The aim of the present study is to explore whether it is possible to subdivide isolates of MRSA CC398, commonly livestock associated, using an enhanced version of the MALDI-TOF MS typing method that we previously described (Ãstergaard et al, 2015). We included MALDI-TOF spectra from 378 isolates of MRSA belonging to CC398, of which 322 were epidemiologically independent. We identified 17 peaks as discriminatorily useful and could therefore reliably subdivide the isolates into 23 subtypes, including a distinct type corresponding to a strain from an unusual and initially undiscovered hospital outbreak. Whole genome sequencing was carried out for 193 of the isolates and compared with both the spa type and an antibiogram of these strains. The proposed MALDI-TOF subdivision method for MRSA CC398 was found to be more discriminative than both spa typing and resistotyping, and had a high negative predictive value for ruling out a close genetic relationship between pairs of strains with different MALDI-TOF types. We conclude that the MALDI-TOF-based typing method can be used for rapid and inexpensive routine subdivision of MRSA belonging to CC398.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 308, Issue 4, June 2018, Pages 476-485
Journal: International Journal of Medical Microbiology - Volume 308, Issue 4, June 2018, Pages 476-485
نویسندگان
Claus Ãstergaard, Jens Kjølseth Møller,