کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8384885 | 1543665 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential daptomycin resistance development in Staphylococcus aureus strains with active and mutated gra regulatory systems
ترجمه فارسی عنوان
توسعه مقاومت دپوتومایسین دیفرانسیل در سویه های استافیلوکوکوس اورئوس با سیستم های تنظیم کننده گره فعال و جهش یافته
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
The first-in-class lipopeptide antibiotic daptomycin (DAP) is highly active against Gram-positive pathogens including Ã-lactam and glycopeptide resistant strains. Its molecular mode of action remains enigmatic, since a defined target has not been identified so far and multiple effects, primarily on the cell envelope have been observed. Reduced DAP susceptibility has been described in S. aureus and enterococci after prolonged treatment courses. In line with its pleiotropic antibiotic activities, a unique, defined molecular mechanism of resistance has not emerged, instead non-susceptibility appears often accompanied by alterations in membrane composition and changes in cell wall homeostasis. We compared S. aureus strains HG001 and SG511, which differ primarily in the functionality of the histidine kinase GraS, to evaluate the impact of the GraRS regulatory system on the development of DAP non-susceptibility. After extensive serial passing, both DAPR variants reached a minimal inhibitory concentration of 31â¯Î¼g/ml and shared some phenotypic characteristics (e.g. thicker cell wall, reduced autolysis). However, based on comprehensive analysis of the underlying genetic, transcriptomic and proteomic changes, we found that both strains took different routes to achieve DAP resistance. Our study highlights the impressive genetic and physiological capacity of S. aureus to counteract pleiotropic activities of cell wall- and membrane-active compounds even when a major cell wall regulatory system is dysfunctional.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 308, Issue 3, April 2018, Pages 335-348
Journal: International Journal of Medical Microbiology - Volume 308, Issue 3, April 2018, Pages 335-348
نویسندگان
Anna Müller, Fabian Grein, Andreas Otto, Kathrin Gries, Dmitriy Orlov, Vladimir Zarubaev, Myriam Girard, Xinwei Sher, Olga Shamova, Terry Roemer, Patrice François, Dörte Becher, Tanja Schneider, Hans-Georg Sahl,