کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8384887 | 1543665 | 2018 | 37 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Performance of a highly successful outbreak strain of Mycobacterium tuberculosis in a multifaceted approach to bacterial fitness assessment
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کلمات کلیدی
DTHOFXextensively-drug resistantIS6110-RFLPPZAXDRrpoBMDMSITMICMDRINHCFUEMBSTRMOI - MEethambutol - اتیامبوتولStreptomycin - استروتومایسینOfloxacin - افلوکساسینisoniazid - ایزونیازیدdelayed-type hypersensitivity - تاخیر نوع حساسیت بالاMinimal inhibitory concentration - حداقل غلظت مهار کنندهRifampicin - ریفامپینMultidrug-resistant tuberculosis - سل مقاوم به چندین داروhuman monocyte-derived macrophages - ماکروفاژهای حاصل از مونوسیت انسانMycobacterium tuberculosis - مایکوباکتریوم توبرکلوزیسMultidrug-resistant - مقاوم در برابر چند داروKan - ممکن استcolony forming units - واحدهای تشکیل دهنده کلنیVirulence - ویروسی شدنPyrazinamide - پریزامینیدmultiplicity of infection - چندین عفونتKanamycin - کانامایسینRif - کد عکس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Determining bacterial fitness represents a major challenge and no single parameter can accurately predict the ability of a certain pathogen to succeed. The M strain of Mycobacterium tuberculosis managed to spread and establish in the community and caused the largest multidrug-resistant tuberculosis outbreak in Latin America. We have previously shown that the M strain can manipulate the host immune response, but we still have no direct evidence, other than epidemiology, that can account for the enhanced fitness of the M strain. Our objective was to further characterize the performance of the outbreak strain M in different fitness assays. Two main aspects were evaluated: (1) molecular characterization of selected isolates from the M outbreak and related strains and (2) comparative fitness and in vivo performance of representative M strain isolates vs. the non-prosperous M strain variant 410. Our approach confirmed the multifaceted nature of fitness. Altogether, we conclude that the epidemiologically abortive strain 410 was vulnerable to drug-driven pressure, a weak competitor, and a stronger inductor of protective response in vivo. Conversely, the isolate 6548, representative of the M outbreak peak, had a growth disadvantage but performed very well in competition and induced lung damage at advanced stages in spite of reaching relatively low CFU counts. Integration of these observations supports the idea that the M strain managed to find a unique path to success.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 308, Issue 3, April 2018, Pages 349-357
Journal: International Journal of Medical Microbiology - Volume 308, Issue 3, April 2018, Pages 349-357
نویسندگان
Noemà Yokobori, Beatriz López, Johana Monteserin, Roxana Paul, Andrea Von Groll, Anandi Martin, Brenda Marquina-Castillo, Juan Carlos Palomino, Rogelio Hernández-Pando, MarÃa del Carmen Sasiain, Viviana Ritacco,