کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8385284 | 1543689 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Global analysis of bacterial membrane proteins and their modifications
ترجمه فارسی عنوان
تجزیه و تحلیل جهانی پروتئین های غشایی باکتری ها و تغییرات آنها
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
Membrane proteins are situated at the interface of bacterial cell and its environment, and are therefore involved in vital physiological processes such as nutrient exchange, signal transduction and virulence. Due to their distinct biophysical properties, especially hydrophobicity, they are difficult subjects to study. Classical proteomics technologies have relied on multidimensional separation of proteins on gels, which largely limited the choice of detergents and made the development of specialized enrichment protocols for membrane proteins necessary. Shotgun proteomic approaches, based on the digestion of whole proteomes and subsequent analysis of peptides by LC-MS, has largely circumvented these problems due to its compatibility with potent detergents. Here we briefly present and discuss the major developments in bacterial membrane proteomics and argue that recent developments in biochemical sample preparation and high resolution mass spectrometry have the potential to comprehensively identify and quantify membrane proteins without the need for specific enrichment procedures prior to LC-MS analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 305, Issue 2, February 2015, Pages 203-208
Journal: International Journal of Medical Microbiology - Volume 305, Issue 2, February 2015, Pages 203-208
نویسندگان
Boumediene Soufi, Boris Macek,