کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8385398 | 1543692 | 2014 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic and transcriptional activities of Staphylococcus aureus challenged with high-doses of daptomycin
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Metabolic and transcriptional activities of Staphylococcus aureus challenged with high-doses of daptomycin Metabolic and transcriptional activities of Staphylococcus aureus challenged with high-doses of daptomycin](/preview/png/8385398.png)
چکیده انگلیسی
Treatment of stationary growth phase Staphylococcus aureus SA113 with 100-fold of the MIC of the lipopeptide antibiotic daptomycin leaves alive a small fraction of drug tolerant albeit genetically susceptible bacteria. This study shows that cells of this subpopulation exhibit active metabolism even hours after the onset of the drug challenge. Isotopologue profiling using fully 13C-labeled glucose revealed de novo biosynthesis of the amino acids Ala, Asp, Glu, Ser, Gly and His. The isotopologue composition in Asp and Glu suggested an increased activity of the TCA cycle under daptomycin treatment compared to unaffected stationary growth phase cells. Microarray analysis showed differential expression of specific genes 10Â min and 3Â h after addition of the drug. Besides factors involved in drug response, a number of metabolic genes appear to shape the signature of daptomycin-tolerant S. aureus cells. These observations will be useful toward the development of new strategies against persisters and related forms of bacterial cells with downshifted physiology.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Medical Microbiology - Volume 304, Issue 8, November 2014, Pages 931-940
Journal: International Journal of Medical Microbiology - Volume 304, Issue 8, November 2014, Pages 931-940
نویسندگان
Sabrina Lechner, Marcel Prax, Birgit Lange, Claudia Huber, Wolfgang Eisenreich, Alexander Herbig, Kay Nieselt, Ralph Bertram,