Genomic differences between the food-grade Staphylococcus carnosus and pathogenic staphylococcal species

By comparative analyses based on the newly sequenced genome of the meat starter bacterium Staphylococcus carnosus TM300, we observed remarkable differences in the content of mobile genetic elements between non-pathogenic and pathogenic staphylococci. While the latter reveal highly flexible genomes with various mobile elements indicating frequent exchange and rearrangement of genomic material, S. carnosus shows a conspicuous lack of those elements carrying only remnants of a prophage and a genomic island in its genome. Furthermore, the S. carnosus genome is significantly poor in repetitive sequences. Despite being known as completely avirulent, S. carnosus reveals also various gene products with similarity to proteins annotated as virulence factors in S. aureus. In addition, the genome carries a number of mutationally inactivated genes including those of the global regulatory systems agr and sae. Our data indicate that S. carnosus has adapted to the constant environmental conditions encountered as part of a starter culture population by a reductive evolution leading to gene loss and inactivation.