کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8393092 | 1544058 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of goose SPMS: Molecular characterization and expression profiling of SPMS in the goose ovary
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
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چکیده انگلیسی
Spermine synthase (SPMS), which converts spermidine into spermine, is essential for normal cell growth and development processes in humans and other mammals, but the molecular characterization and expression profiling of the SPMS gene remain undetermined in goose tissues and ovarian follicles. In this study, the SPMS cDNA sequence of the Sichuan white goose was cloned and analysed, and SPMS mRNA expression was profiled in various tissues and ovarian follicles. The results showed that the open reading frame of the SPMS cDNA sequence was 1092â¯bp in length, encoding 363 amino acids with a molecular weight of 41â¯kDa. Among all the examined tissues, SPMS expression was highest in the spleen and cerebrum and lowest in the breast and thigh muscles. SPMS expression in the F1 follicle was significantly higher than that in the POF (except for POF2) (Pâ¯<â¯0.05). Our results indicate that SPMS might play an important role in follicular development and ovulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Reproductive Biology - Volume 18, Issue 1, March 2018, Pages 60-65
Journal: Reproductive Biology - Volume 18, Issue 1, March 2018, Pages 60-65
نویسندگان
Bo Kang, Qilin Xu, Ziyu Chen, Yongsheng Wu, Su Yang, Xicheng Yang, Zhao Zhang, Dongmei Jiang,