The production and characterization of anti-bothropic and anti-crotalic IgY antibodies in laying hens: A long term experiment

► We verified the neutralizing activities of the IgY antibodies. ► When inoculated with crotalic or bothropic venoms, hens produce high levels of IgY. ► In a long-term experiment, IgY present higher avidity. ► The high avidity of the IgY provides high neutralizing activity.