Preclinical assessment of a polyspecific antivenom against the venoms of Cerrophidion sasai, Porthidium nasutum and Porthidium ophryomegas: Insights from combined antivenomics and neutralization assays

► The neutralization of the venoms of species of the Porthidium group was tested. ► Antivenom neutralized toxic and enzymatic activities of the venoms. ► Extensive paraspecific immunoreactivity was detected by immunoblotting. ► Antivenomics showed a variable pattern of immunoreactivity.