کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8425123 | 1546023 | 2018 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lymphoblast-derived integration-free iPSC line AD-TREM2-3 from a 74â¯year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Lymphoblast-derived integration-free iPSC line AD-TREM2-3 from a 74â¯year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant Lymphoblast-derived integration-free iPSC line AD-TREM2-3 from a 74â¯year-old Alzheimer's disease patient expressing the TREM2 p.R47H variant](/preview/png/8425123.png)
چکیده انگلیسی
Human lymphoblast cells from a male diagnosed with Alzheimer's disease (AD) expressing the TREM2 p.R47H variant were used to generate integration-free induced pluripotent stem cells (iPSCs) by over-expressing episomal-based plasmids harbouring OCT4, SOX2, KLF4, LIN28, L-MYC and p53 shRNA. The derived iPSC line - AD-TREM2-3 was defined as pluripotent based on (i) expression of pluripotency-associated markers (ii) embryoid body-based differentiation into cell types representative of the three germ layers and (iii) the similarity between the transcriptome of the iPSC line and the human embryonic stem cell line H1 with a Pearson correlation of 0.940.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Stem Cell Research - Volume 30, July 2018, Pages 141-144
Journal: Stem Cell Research - Volume 30, July 2018, Pages 141-144
نویسندگان
Soraia Martins, Hatice Yigit, Martina Bohndorf, Nina Graffmann, Aurelian Robert Fiszl, Wasco Wruck, Kristel Sleegers, Christine Van Broeckhoven, James Adjaye,