کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456279 | 1548569 | 2016 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
DNA damage and repair kinetics of the Alternaria mycotoxins alternariol, altertoxin II and stemphyltoxin III in cultured cells
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کلمات کلیدی
FPGDNA single strand breakATX IISSBHprtDSBFCSAMEAOHDMEMPBSapurinic/apyrimidinic - apurinic / apyrimidinicDMSO - DMSONER - DOWNDulbecco’s modified Eagle medium - Modified Eagle اصلاح شده Dulbeccoalternariol - آلترنالیونnucleotide excision repair - تعمیر مجدد نوکلئوتیدیDimethyl sulfoxide - دیمتیل سولفواکسیدfetal calf serum - سرم گوساله جنینDNA double strand break - شکست دو رشته DNAFormamidopyrimidine-DNA glycosylase - فرمامینیدوپیریمیدین DNA گلیسوزیلازPhosphate buffered saline - فسفات بافر شورhypoxanthine guanine phosphoribosyl transferase - هیپوکسانتین گوانین فسفریبوسیل ترانسفرازplating efficiency - کارآیی پوشش
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
DNA double strand breaks induced by AOH due to poisoning of topoisomerase IIα were completely repaired in less than 2 h. Under cell-free conditions, inhibition of topoisomerase IIα could also be measured for ATX II and STTX III at low concentrations, but the perylene quinones were catalytic inhibitors rather than topoisomerase poisons and did not induce DSBs. DNA strand breaks induced by ATX II and STTX III were more persistent and not completely repaired within 24 h. A dependence of the repair rate on the NER status could only be demonstrated for STTX III, resulting in an accumulation of DNA damage in NER-deficient cells. Together with the finding that the DNA glycosylase formamidopyrimidine-DNA glycosylase (Fpg), but not T4 endonuclease V, is able to generate additional DNA strand breaks measurable by the alkaline unwinding assay, we conclude that the genotoxicity of the perylene quinones with an epoxide group is probably caused by the formation of DNA adducts which may be converted to Fpg sensitive sites.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 798â799, March 2016, Pages 27-34
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 798â799, March 2016, Pages 27-34
نویسندگان
Stefanie C. Fleck, Friederike Sauter, Erika Pfeiffer, Manfred Metzler, Andrea Hartwig, Beate Köberle,