کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8456413 | 1548582 | 2015 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Repeated-dose liver micronucleus assay: An investigation with 2-nitropropane, a hepatocarcinogen
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کلمات کلیدی
DAPIHepatocarcinogenHepS2-Nitropropane - 2-نیترو پروپان4′,6-diamidino-2-phenylindole dihydrochloride - 4 '، 6-دی آمیدین-2-فنیلینول دی هیدروکلرایدIME - NAMELiver micronucleus assay - آزمایش میکروسکوپی کبدیBone marrow micronucleus assay - آزمایش میکرونوکلئوس مغز استخوانAcridine orange - آکاردین نارنجیRepeated-dose toxicity study - مطالعه سمیت دوزهای مجزا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Repeated-dose liver micronucleus assay: An investigation with 2-nitropropane, a hepatocarcinogen Repeated-dose liver micronucleus assay: An investigation with 2-nitropropane, a hepatocarcinogen](/preview/png/8456413.png)
چکیده انگلیسی
The utility of the repeated-dose liver micronucleus (RDLMN) assay in the detection of a genotoxic hepatocarcinogen was evaluated. In this paper, a rat hepatocarcinogen, 2-nitropropane (2-NP), was administered orally to young adult rats for 14 and 28 days without a partial hepatectomy or a mitogen, and the micronucleus induction in liver was examined using a simple method to isolate hepatocytes. In addition, a bone marrow micronucleus assay was conducted concomitantly. The frequency of micronucleated hepatocytes induced by 2-NP increased significantly in both the 14- and 28-day repeated-dose studies, while the bone marrow micronucleus assays were negative in each study. These results indicate that the RDLMN assay is useful for detecting a genotoxic hepatocarcinogen that is negative in bone marrow micronucleus assays and is a suitable in vivo genotoxicity test method for integration into a repeated-dose general toxicity study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 780â781, March 2015, Pages 60-63
Journal: Mutation Research/Genetic Toxicology and Environmental Mutagenesis - Volumes 780â781, March 2015, Pages 60-63
نویسندگان
Satoru Kawakami, Tetsuro Araki, Mikio Nakajima, Osamu Kusuoka, Keisuke Uchida, Norihiro Sato, Yoko Tanabe, Kaori Takahashi, Yumi Wako, Kazufumi Kawasako, Kazuyuki Tsurui,