Development and validation of a high-content screening in vitro micronucleus assay in CHO-k1 and HepG2 cells

► In drug development assays for the early detection of genotoxicity are needed. ► Two high content screening (HCS) micronucleus assays were developed and validated. ► Both HCS assays in HepG2 cells and CHO-k1 cells were able to detect genotoxic potential. ► The assays gave a low number of false-positive results. ► The assays allow differentiation into clastogens and aneugens.