کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8472078 | 1550291 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae Identification of a novel cofilin-related molecule (Der f 31) as an allergen from Dermatophagoides farinae](/preview/png/8472078.png)
چکیده انگلیسی
House dust mite (HDM) allergen is a major cause of allergic disease. In this study, two-dimensional immunoblot and Matrix-Assisted Laser Desorption Ionization tandem Time-of-flight mass spectrometry (MALDI-TOF-MS) were used to identify Der f 31. After Der f 31 was cloned, expressed and purified, skin prick test (SPT), Immune inhibitory assays, Western blot, ELISA and asthmatic mouse model were employed to examine the allergenicity of recombinant Der f 31. The gene of Der f 31 includes 447 bps, and encoded 148 amino acids. Positive responses of SPT to r-Der f 31 were 32.5% in 43 HDM-allergic patients. r-Der f 31 can induce allergic pulmonary inflammation in the mouse model. In conclusion, Der f 31 is a novel subtype of dust mite allergens.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 223, Issue 2, February 2018, Pages 246-251
Journal: Immunobiology - Volume 223, Issue 2, February 2018, Pages 246-251
نویسندگان
Jianli Lin, Nana Huang, Hui Wang, Qinghui Fu, Eryi Wang, Ping Li, Liteng Yang, Xinping Luo, Xiaoyu Liu, Zhigang Liu,